- 多克隆位點
- multiple: adj. 1.多重的；復合的,復式的,多數(shù)的,多樣的。 ...
- clone: n. 1.【生物學】純種細胞，無性系。 2.復制品；(幾 ...
- site: n. 1.地點；位置；地基。 2.場所，現(xiàn)場。 3.遺址 ...
- multiple cloning site: 多克隆位點
- multiple combining site: 多結合位點
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multiple clone site中文什么意思
- 多克隆位點
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例句與用法
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- According to csfv ' s e2 gene sequence published in genbank and the sequence of p . pastoris expression vector ppic9 ' s multiple cloning site ( mcs ) , a pair of primers were designed with oligo and primers . o softwares
將該基因片段先克隆到pmd18 - t載體上，并進行了酶切、 pcr鑒定和測序分析，陽性重組質粒命名為t - e2 。 - Construction of two vectors containing different plasmid original replicon this work constructed four resolution shuttle vectors , pbmb1205 , pbmb1205r , pbmb1206 and pbmb1206r . there are multiple clone sites between two copies of res sites
解離載體的構建和性能利用來源于不同質粒上的質粒復制起始區(qū)ori44和ori1030構建了4個解離載體。 - The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak . 8 . bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
將克隆到的hbmp基因通過適當?shù)拿盖胁迦氲睫D移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。 - The interest gene was inserted in the - tha l . tho 1 multiple cloning sites of donor plasimd pfastbachtb of baculovirus expression system . after analysis by restriction endonuclease and pcr , the recombinant donor plasmid gpl - fast was transforn1ed to the competent celi dhi0bac which contalns the bacmid and the helper plasmid , the recombinant bacmid gpl - bac was acquired which would express the vpl of gpv strain h l
同時將該目的基因插入到桿狀病毒表達系統(tǒng)的供體質粒pf _ ( ast ) b _ ( ac ) htb的xba 、 xho多克隆位點間，經酶切、 pcr鑒定后，將重組的供體質粒gp1 - fast轉化到含有桿狀病毒和輔助質粒的dh10b _ ( ac )感受態(tài)細胞中，獲得了表達gpvh1株vp1的重組桿狀病毒gp1 - bac 。 - A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally
進一步序列分析表明該復制區(qū)至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續(xù)培養(yǎng)72h ，復制區(qū)質粒在bt無晶體突變株hd73cry ~ -中穩(wěn)定性達98以上， 30h生長曲線也表明該復制區(qū)能夠在bt中穩(wěn)定復制和遺傳，對受體菌株無明顯不良影響。
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